Confocal Scanning fluorescence Detection System and its Optimization

摘要

Fluorescence detection by confocal scanning systems has advantages of large area detection, small focus depth, large numerical aperture, complete laser rejection, low background noise, high snesiitivty and S/N ratio. It is a sensitive and precise method to record and analysis results of biological or biomedical assay, such as DNA hybridization assay or micro capillary electrophoresis assay on biochip. Parameters like power of laser or sensitivity of PMT are responsible for signal to noise ratio and sensitivity of the system, which should be optimized relative to different situations. The structural design and experimetnal analysis on parameter optimization of a confocal scanning fluorescence detection system are presented in this paper.