Novel microfabricated capillary array electrophoresis chip fabricated by synchrotron radiation and direct observation of dynamics of DNA molecules migrating in microchannels

摘要

We have examined the new technology for the microfabrication of capillary array electrophoresis (CAE) chip using synchrotron radiation lithography in polymethylmethacrylate (PMMA) substrates and developed novel type microfabricated CAE chip. Laser-induced fluorescence detection system for the CAE chip has been constructed by the modification of laser confocal fluorescence microscopy. DNA molecules labeled by intercalating fluorescence dyes was detected during migrating into the array of microchannels and conformational dynamics of single DNA molecule in microchannels filled with linear polyacrylamide under electric field was observed by this system to elucidate the separation mechanism of DNA and to optimize the separation conditions for DNA analysis. BAsed on this observation, we demosntrate that linear polyacrylamide is suitable for the rapid separation of large DNA fragments more than 100 kbp by microfabricated CE chip without pulsed electric field.