IMMUNOCHEMICAL ASSAYS FOR PESTICIDES AND PCBs

摘要

Analytical parameters of any immunochemical assay are based primarily on binding characteristics of the antibody employed. In this laboratory, a wide variety of immunogens, antibodies and tracer conjugates have been developed to incorporate them into competitive enzyme linked immunoadsorbent assays (ELISAs) and other immunochemical formats including biosensors. The antibody based assays, working on competitive principle, provided sensitive responses to the presence of ppt-ppb amounts of toxic substances measured in terms of changes of radioactive, color, fluorescent, or electric signals. Thus, a sensitive and highly selective immunochemical analysis of 2,4-dichlorophenoxyacetic acid (2,4-D), s-triazines and polychlorinated biphenyls (PCBs) can be performed under both laboratory and field conditions. Favorable correlation between immunochemical and instrumental techniques has been found. Several interrelated issues concerning the conventional immunoassays and immuno-sensors are compared and discussed in terms of needs and methodological maturation.