LAYERED DOUBLE HYDROXIDE: A NEW CERAMIC-BASED HEMOSTATIC AGENT?

摘要

Layered double hydroxides (LDHs), well known as anionic clays or hydrotalcite-like compounds consist of positively charged layers. Net positive charges on the layers are balanced by exchangeable anions along with water molecules in the interlayer space. In the present research, the potential for use of Ca/Al-LDH as local hemostatic agent is evaluated in vitro by measuring the coagulation time of human fresh blood in the presence of LDH powder. Crystalline Ca/Al-LDH powders required for these experiments were synthesized via a co-precipitation method followed by a controlled hydrothermal process using calcium nitrate and aluminum nitrate as starting materials under certain synthetic conditions such as time and concentration. XRD and SEM analyses were utilized to characterize the synthetic powders. XRD and SEM results demonstrate the presence of a crystalline pure Ca/Al-LDH with hexagonal morphology. In order to investigate the biocompatibility of the samples, culture techniques were employed using mouse fibroblast cells (L929) as reference cell line. The results reveal that LDH powder can accelerate the platelet aggregation and the formation of blood clot. Moreover, the samples show good cell proliferation and high cell viability to L929 cell line compared with negative control sample. These results demonstrate that these layered materials are suitable candidates for using as local hemostatic agent to hemorrhage control and can reduce blood loss in lethal injuries.