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Multimodal optical detection and toxicity testing of microplastics in the environment

机译:环境中微塑料的多模式光学检测和毒性测试

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Microplastics are small plastic particles the size of less than 5 millimeters from cosmetics or results of abrasion anddecomposition of plastic waste. The tremendous marine pollution by plastic particles and fibers and the increasingpresence in the human environment from drinking water reservoirs to waste water demands for an environmentalmanagement and effective detection methods. The uptake of microplastics by living organisms may cause injuries of thegastrointestinal tract, trigger inflammation or cause cell toxicity by intrinsic particle properties or adsorbed pollutants.The urgent need for methods to identify microplastics in the environment, its sources of input and the risk of microplasticparticles is the objective of the research project MicroPlastiCarrier.The project develops new tools for the optical detection and identification of microplastic particles from wastewater by amultiwavelength approach. The multiple labelfree optical toolbox is based on digital holographic microscopy usingwavelengths from the visible to mid infrared. In order to monitor particle uptake minimally-invasively in livingorganisms and cellular specimens in a label-free manner, we applied high resolution optical coherence tomography(OCT) and multi-spectral digital holographic microscopy (DHM).In combination with microfluidics technologies as flow cytometry the project plans to identify particles based on sizeand their absorption and refraction index properties at several wavelengths. The technology should overcome thelimitations of state of the art FT-IR.
机译:微塑料是小塑料颗粒的尺寸小于5毫米的化妆品或磨损的结果塑料废物的分解。塑料颗粒和纤维的巨大海洋污染和增加从饮用水储层的人类环境中的存在,以浪费环境的需求管理和有效的检测方法。生物体的微薄塑料吸收可能导致伤害通过内在颗粒性质或吸附的污染物,胃肠道,引发炎症或引起细胞毒性。迫切需要方法以鉴定环境中微薄塑料,其输入来源和微塑料的风险粒子是研究项目微生物载体的目的。该项目开发了用于光学检测和识别来自废水的新工具多波长方法。多个Labelfree光学工具箱基于数字全息显微镜使用从可见的波长到中红外线。为了监测粒子摄取最微弱的生活有机体和细胞样本以标记的方式,我们应用了高分辨率光学相干断层扫描(OCT)和多光谱数字全息显微镜(DHM)。与微流体技术相结合,作为流式细胞仪,项目计划识别基于尺寸的粒子及其在多个波长下的吸收和折射率特性。该技术应该克服FT-IR状态的局限性。

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