Although both of raloxifene glucuronides were oxidized electrochemically, the electrolytic potential for oxidation of raloxifene 4'-glucuronide was lower than that of raloxifene 6-glucuronide. The fragmentations of these electrochemical oxidation product ions were anlayzed in both positive and negative ion modes, and it was presumed that the site of electrochemical oxidation in the chemical structure of raloxifene 4'-glucuronide would be in the phenyl ring of benzothiophene moiety. It was suggested that on-line EC/ESI-MS~n technique would be useful for estimating the metabolic site of glucuronidation in drug discovery if the unchanged drug has phenolic hydroxyl group.
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