Development of Capillary Electrophoresis-Mass Spectrometry for Analysis of Fenton Chemistry Biomass Pretreatment for Biofuels Production

摘要

Although, thioacidolysis monomeric products were not electrophoretically resolved, families of compounds were resolved enhancing mass spectral elucidation. A variety of these compounds were putatively identified as monomeric and β-O-4 linkage derived compounds. After fenton treatment, monomeric and β-O-4 linkage were reduced in the solid reaction product (relative to untreated reaction product), however these compounds were observed in the filtrate, suggesting these compounds were soluble products of the fenton pretreatment. The ability of CE-MS to detect other compounds from lignin thioacidolysis, especially compound derived from the linkages that compose 50% of lignin will allow for more complete characterization of lignin and the affects of fenton chemistry on lignin. Currently, CE-MS has shown to be comparable to GC-MS in detecting monomeric ratios and was more sensitive. CE-MS provides the ability to simultaneously detect the building blocks of lignin and products of fenton reaction pretreatment; this provides a more comprehensive analytical method in order to understand fenton chemistry and its affects on the lignin structure.