Evaluation of High Resolution Accurate Mass Spectrometer for Bioanalytical Support of in vitro ADME Screening Assays

摘要

Utilization of high resolution accurate mass spectrometry completely eliminated MRM compound optimization. No dwell time was involved in full scan using HRAM, maintaining data quality while performing simultaneous acquisition of cassette samples. Cassette incubation reduced number of assay samples and the subsequent sample analysis time. Cassette incubation generates similar protein binding results to individual incubation, while effectively reducing the biological assay cost in usage of reagents, plates and assay runtime.