Quantitative Analysis of 6-Thioguanine-induced Changes in the Proteome of Jurkat-T Human Leukemia Cells

摘要

Acute lymphoblastic leukemia (ALL) is the leading cause of cancer-related deaths in children. There is a pressing need for developing novel and effective therapeutic avenues for ALL treatment, thereby improving the cure rate and the quality of life of ALL patients. Thiopurine drugs, including 6-thioguanine (~(S)G) and 6-mercaptopurine, are among the most widely prescribed drugs for ALL treatment, though their mechanisms of action remain unclearly defined. Herein, we employed metabolic labeling with SILAC (stable isotope labeling by amino acids in cell culture), together with LC-MS/MS to assess quantitatively the perturbation of protein expression in cultured human cells upon ~(S)G treatment. Our objective is to gain insights into the cytotoxic mechanisms of ~(S)G.