Rapid automated off-line LC-MALDI analyses using a liquid matrix optimized for uniformity and durability of ionization

摘要

One concern in LC-MALDI is amount of time dedicated to the raster scanning function during automated analyses on crystalline solid matrices such as alpha-cyano-4-hydroxycynamic acid (alpha- CHCA). We report here a rapid automated off-line LC-MALDI analysis method using an optimized liquid matrix alpha-CHCA butylamine salt (CHCAB) for uniformity and durability of ionization. Just 1-3 discrete positions on each sample spot were irradiated with a laser for MS and MS/MS. As a result, bovine serum albumin (BSA), alcohol dehydrogenase (ADH) and chicken lysozyme (LZM) were automatically identified using optimized CHCAB in one-third or one-quarter of the total analytical time required using alpha-CHCA.