EFFECT OF GENOTYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION IN SUGARCANE

摘要

Young leaf bit explants of three sugarcane species (S. officinanim. S. barberi. S. sinense). one intergeneric hybrid (Saccharum x Zeal and five varieties (Co 86032, Co 99004, Co 94012, Co 1148 and CoJ 64) was asepticaUy inoculated on MS medium containing different concentration of 2, 4-D (0, 1.0, 2.0, 3.0, 4.0 mg/l)for callus induction. Maximum of 95% callus induction was observed in the variety Co 99004 and Saccharum x Zea showed lowest callus induction (40%). The actively growing calli were transferred to MS medium supplemented with different concentrations of 6-Benzyl Amino Purine (BAP), kinetin and combination of BAP and kinetinfor shoot regeneration. Most of the genotypes showed maximum shoot regeneration in MS medium supplemented with a combination of BAP and kinetin (0.5 mg/l each). S. officinarum and Co 86032 showed maximum number of regenerated shoots in medium supplemented with 0.5 mg/lBAP + 0.5 mg/l kinetin and 1 mg/l kinetin respectively. From the study it was concluded that callus induction and shoot regeneration is significantly affected by genotype and the growth regulators.