The use of autologous stem cells CD133 + for regenerative treatment of cardio-vascular diseases.

摘要

Objective: To study the safety and effectiveness of transplantation of autolog stem cells CD133, isolated from the bone marrow of the patients.Material and methods: The study included 33 patients: 13 patients (12 male and 1 female, mean age 52 ± 8 years) had ischemic heart disease (IHD), angina of I-III functional class (FC), with a anamnesis the Q-rnyocardial infarction prescription 3 -6 months, post-infarction cardiosclerosis, 8 (4 males and 4 females, mean age 33 ± 8 years) had a liver cirrhosis (LC) and 12 have an obliterative disease of the lower extremities. Bone marrows were taken as standard puncture of the sternum under local anesthesia. Stem cells CD133 (SC-CD133) were isolated by density gradient centrifugation in ficoll, followed by irnmuno-magnetic separation. Autologous isolated cells of patients (5 ml of suspension containing 0,8-L5 million SC-CD133) were used for each patient: in those with coronary artery disease the cells were injected intraar-terially into the coronary arteries under angiography, in patients with liver cirrhosis the cells were injected into the hepatic vein, and in patients with obliterative diseases of the lower extremities, the cells were injected into the common femoral artery. All patients received 0.8mg estradiol/day for two months.Results: After transplantation, in patients with IHD, ejection fraction of left ventricle increased from 50 ± 9% to 58 ± 9% (p <0,001) after 6 months in addition noted the decrease in LV cavity according scintigraphy. Positive dynamic parameters were also observed in patients with obliterative diseases of the lower extremities, the saturation of tissue oxygenation in the affected limb in most patients has improved significantly. The results of cell therapy in patients with LC are still in the process of learning.Conclusion: The procedure for implantation of autologous stem cells CD133,isolated from the bone manow of the patients is safe, has a beneficial effect on the process of rehabilitation treatment of various organs.