Breeding of a stable CMS line without trace-pollen by means of improving Pol CMS line in Brassica napus L.

摘要

The occurrence of trace-pollen is a serious problem existing widely in CMS lines of rapeseed. To breed stable ms line without trace-pollen, a breeding program for improving Pol CMS line using recessive genic ms line and thermo-sensitive genic ms line had been carried out since 1991. A new stable CMS line, named as Xiangyou66A, had been bred successfully. The investigations to the fertility, economic traits and quality of Xiangyou66A showed that it was very stable in different years with 100% completely sterile plants and it had fine economic traits and double-lowed quality. The identification trial indicated that Xiangyou66A had the same restoring-maintaining relationship as the existing Pol CMS lines. Seven restores which can completely restore the fertility of Xiangyou66A were selected out. The combinations using these restorers crossed with Xiamgyou66A displayed different heterosis in the variety comparing trial. Six of them over-yielded with the increasing rate of 2.28%～19.21% compared with the check, among which three combinations had the increasing rate more than 10%.