Region- and enzyme-specific bioconversion of dynorphin neuropeptide detected by in situ histochemistry and MALDI imaging mass spectrometry.

摘要

Here we developed a new method combining in situ histochemistry (ISH) with MALDI imaging MS (ISH-MALDI imaging MS) for the analysis of bioconversion of neuropeptides. The aim of our first experiments was to determine the effect of specific enzyme inhibitors on dynorphin bioconversion in different brain regions. ISH-MALDI imaging MS has several unique advantages that are interesting for anyone developing new pharmaceuticals involving enzymatic reactions: 1. Substrate and products are measured simultaneously. 2. Region- and enzyme-specific reactions can be detected.