A STUDY ON PROTEIN OXIDATIVE DAMAGE IN MICE INDUCED BY GASEOUS FORMALDEHYDE

摘要

To explore protein oxidative damage induced by gaseous formaldehyde and its mechanism we carried out this study. Mice were treated with gaseous formaldehyde at different levels (0mg.m-3, 0.68mg.m-3 and 3.0mg.m-3) for 72 hr and the protein carbonyl content was mensurated using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage. The results show that the protein carbonyl content of brain, heart and liver tissue in mice decrease in the group of0.68mg.m-3 (p＜0.01, p＜0.01, p＜0.05), while increase significantly in heart and liver tissue in the group of 3.0mg.m-3 (p＜0.01, p＜0.01) and protein carbonyl content in brain does not have notable difference compared with the control (p＞0.05). These results indicate that the protein oxidative damage induced by gaseous formaldehyde depends on the concentration of gaseous formaldehyde. Medium concentration formaldehyde may not induce protein oxidative damage, while high concentration formaldehyde can cause protein oxidative damage in heart and liver tissue markedly and has little effect on protein of brain.