Effect of Reduction in Ammonium Nitrate of Murashige and Skoog Medium on In Vitro Rooting from the Shoots of Some Horticultural Plants

摘要

The formation of adventitious roots in vitro is very important for obtaining good transplants by micropropagation. Horticultural plants differ greatly in rooting ability in vitro. In some plants' the shoots are capable of rooting easily in hormone-free medium. In other plants, auxins such as naphthaleneacetic acid (NAA) or indolebutyric acid (IBA) are necessary for in vitro rooting. On the other hand, it has been our experience that a reduction in the concentration of an inorganic salt in the mediumpromotes the rooting from the shoots in vitro. For example, in An-thurium (George, 1996) and apple trees [Sriskandarajah et al.l990] a reduction in ammonium nitrate of Murashige and Skoog, (1962), (MS) medium promoted in vitro rooting from the shoots.