Phenanthrene was selected as an hazardous and widespread poly aromatic hydrocarbon. Biodegradation of phenanthrene by a type of fungi was studied. A suitable medium, SM was utilized for growing microorganism and phenanthrene was used as the sole source of carbon and energy in this culture. The effect of phenanthrene concentration and other factors on biodegradation were studied, and the amount of degradation was measured by high performance liquid chromatography. High concentration of phenanthrene was toxic for microorganism in aqueous media, but results of biodegradation in low concentration of phenanthrene was acceptable. Phenanthrene solved in none aqueous phase liquid (NAPL) like hexadecane could not be degraded properly. Surfactant, triton X-100 showed inhibitory effect on phenanthrene biodegradation and fungal growth. Other low molecular weight hydrocarbons such as naphthalene inhibited the phenanthrene degradation.
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