An experimental set-up (batch and dynamic tests) was designed to determine the TNT biodegradation for diverse soils, TNT concentrations, environmental conditions, and the contribution of soil washing and biodegradation to the overall TNT removal during remediation. The contribution of biodegradation was almost twice as high under anaerobic compared to aerobic conditions. About 55.3% and 28.3% of TNT was biodegraded aerobically at 20 and 10oC, respectively, as compared to 99.7% at 20oC, and 99.3% at 10oC, under anaerobic conditions. Biodegradation rates of 1.32-1.52 mg TNT/kg?h under anaerobic conditions during phase 1 were reduced to 0.025-0.03 mg/kg?h in phase 2. The aerobic biodegradation rates were of 0.044-0.063 mg TNT/kg?h. Aerobic biodegradation was much more temperature dependent than the anaerobic process. In the case of the aerobic process, the lowest biodegradation was observed at 10oC, and the process lasted only for 41 hours. The most effective TNT biodegradation was found at 30oC, as indicated by high O2 consumption and CO2 production rates of ca. 458 and 676 μL/h, respectively. The results may be used as a first screening for selection and developing of effective bioremediation strategy for TNT contaminated soils.
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机译：设计实验组（分批和动态测试）旨在确定针对各种土壤，TNT浓度，环境条件的TNT生物降解，以及土壤洗涤和生物降解对整体TNT在修复过程中的贡献。与有氧病症相比，生物降解的贡献几乎是厌氧下的两倍。约55.3％和28.3％的TNT分别在20和10℃下均匀地生物降解，而在厌氧条件下为99.7％，99.3％，99.3％，在厌氧条件下。在阶段1期间厌氧条件下的生物降解率为1.32-1.52mg TNT / kgΔH，阶段2中的厌氧条件下降至0.025-0.03mg / kg = H = H。有氧生物降解率为0.044-0.063mg TNT / kg'h。有氧生物降解比厌氧过程更温度。在有氧过程的情况下，在10℃下观察到最低生物降解，并且该过程仅持续41小时。最有效的TNT生物降解在30℃下发现，如高O2消耗量和CA的CO2生产率所示。分别为458和676μl/ h。结果可以用作TNT受污染土壤的有效生物修复策略的选择和发展的第一筛选。
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