INHIBITION OF BACTERIAL ATTACHMENT TO KIDNEY EPITHELIAL CELLS USING THYMOQUINONE

摘要

Lancefield group A streptococci consists of a Streptococcus pyogens (GAS), which is associated with variety of superlative infections. GAS has the capacity to trigger post infectious syndromes of acute rheumatic fever and post streptococcal glomerulonephritis. GAS is one of the most common human pathogens, and is the most frequent cause of acute pharyngitis (strep throat). GAS can progress very rapidly and lead to soft tissue necrosis, acute kidney failure, adult respiratory distress syndrome, disseminated intravascular coagulopathy, and multi-organ failure and ultimately shock and death [1]. The purpose of this study was to determine if pre-treating or post-treating RMKEC with TQ would alter GAS's ability to adhere to the host cells. Results: The findings from our study indicate that the cells pretreated with TQ (10μM, 50μM, or 100μM) decreased the number of bacteria that were able to attach to the cells in a dose dependent manner. The cells post treated with TQ (10μM, 50μM, or 100μM) decreased the number of bacteria that were able to attach to the cells in a dose dependent manner and was found to be directly proportional to the TQ concentrations. The administration of TQ 10μM was the most effective in preventing the attachment of GAS to pre-treated and post-treated epithelial cells in vitro.