Soft lithography for oligonucleotide arrays fabrication

摘要

A method for fabricating high-density oligonucleotide arrays directed by a set of molecular stamp was reported in this paper. In this method, based on the standard phosphoramidites chemistry protocol, the coupling was conducted under soft lithography. A 20-mer DNA microarray with 10000 probes has been successfully fabricated, which shows the advantages of accurate, reliable operation and low-cost production of high-density DNA chip.