Enzyme electrodes and/or reactors for the determination of lysine content in food proteins, lactulose in milk and glycerol in wine have been developed. The general strategy consisted in the covalent immobilization of enzymes as glycerol-3-P oxidase, glycerokinase, lysine oxidase, fructose dehydrogenase and β-galactosidase onto a membrane or glass beads with electrochemical detection of the reaction products. A Flow Injection Analysis manifold was used for lysine and glycerol while microdialysis sampling was used for lactulose. This work presents data about the analytical optimization of the biosensors as well as application to real samples analysis.
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