Design of Flow Channel for Cell Sorter by Dielectrophoresis with Photolithography Technique

摘要

The flow channel for cell sorter in vitro has been designed by dielectrophoresis with photolithography technique. A pair of asymmetric surface electrodes of titanium (thickness of 200 nm) were incorporated just before the branching flow channel: a triangular electrode with the tip angle of 0.35 rad, and a rectangular electrode of the flat edge as reference. The cyclic alternating electric current of the square wave (between 0.25 μs and 0.3 μs of periods) was introduced between the surface electrodes. The suspension of C2C12 (mouse myoblast cell line) was injected into the flow channel, and the flow rate was controlled by the pressure head between the inlet and the outlet. Experimental results of the pilot test show that the absolute value of the amplitude of the acceleration by the electric field, which is perpendicular to the flow direction, increases with the radius of the cell.