A high performance probe for the study of heterogeneity structureof the photosynthetic apparatus

摘要

Extensive research over the last few years in the domain ofphotobiology has been focused on the structure and function of thephotosynthetic complexes in the thylakoid membrane. The number of phasesin fluorescence induction as the identification of the possibleheterogeneities of photosystem II are debated. Until now theinstrumentation used to measure the fluorescence induction doesn'tpermit an accurate determination of all photosynthetic parameters in thefirst millisecond of the induction. Moreover, the usual methods ofanalysis used to find the number of phases in the fluorescence inductionpresented numerical problems including the lack of resolution. In thisstudy the authors show that the use of the newly developed transparentsubstrate AlInGaP light-emitting diode as a light source permits anaccurate determination of variable fluorescence kinetics. In thisreport, the authors proved also that the use of the GeneralizedPade-Laplace method permitted the resolution of several exponentialswithout any hypothesis on the components number. The experimental testshave shown that the authors' instrumentation provides the advancedfeatures needed for an accurate measurement of fluorescence transientsuseful to study the heterogeneity structure of the photosyntheticapparatus