Design of Electrochemical Nanobiosensor in the Diagnosis of Prostate Specific Antigen (PSA) Using Nanostructures

摘要

In This study, we examine the method of diagnosing prostate cancer, which is one of the most common cancers among men in the world. Prostate-specific antigen (PSA) is the most common marker of prostate cancer, and this study aimed to detect this biomarker by electrochemical nanobiosensor based aptamer using nanostructures Graphene Oxide/Graphitic Carbon Nitride/Gold nanoparticles (GO/G-C₃N₄/Au NPs). The aptamer chains are stabilized on the surface of a glassy carbon electrode (GCE) by Reduced Graphene Oxide, Graphitic Carbon Nitride and Gold nanoparticles (RGO/G-C₃N₄/Au NPs). To ensure the correct operation of the aptamer, a selectivity analysis was taken between five substances C₆H₁₂O₆, BSA, CA15-3, FBS, and PSA, and an electrochemical biosensor designed with good stability and high selectivity, diagnosis the desired analyte (PSA) compared to other materials. For Characterization of aptasensor Electrochemical, Cyclic voltammetry (CV) and square wave voltammetry (SQW) tests were performed to investigate the features of the synthesized nanoparticles, XRD, FTIR tests were carried out and the results indicated that the used nanoparticles were well synthesized. The limit of detection (LOD) is 1.67 pg/ml in hexafrrocyanide ([Fe (CN)6]-3/-4) media.