Fluorescence quenching of bioactive molecules by nanodiamonds

摘要

In this work, the fluorescence quenching of two types of bioactive molecules – of the protein lysozyme and of the drugdoxorubicin – by carboxylated detonation nanodiamonds in the result of their interaction has been studied. It wasdemonstrated that nanodiamonds effectively quench the fluorescence of lysozyme and doxorubicin but by differentmechanisms. It was found that the fluorescence quenching of lysozyme by nanodiamonds is caused only by a static typeof quenching while the fluorescence quenching of doxorubicin by nanodiamonds is caused by both static and dynamictypes of quenching. We propose a hypothesis that the surface groups of nanodiamonds are the quenchers of thefluorescence and the variety of surface groups with which a fluorescent molecule interacts determines the fluorescencequenching mechanism. The accounting of our results will provide the insight in the nanodiamonds’ visualization as wellas the possible way to track the loading and subsequent unloading of drugs from the nanodiamonds’ surface.