A novel gravity valve and its application in a 3D printed centrifugal fluidic-system for solid phase extraction (SPE)

摘要

A 3D centrifugal cartridge with gravity valve, capillary valves, and Coriolis switching valve was designed, fabricated,and tested for Solid Phase Extraction (SPE). In particular, the novel three-dimensional gravity valve helps to releasereagents in not-sequentially increased rotation speeds. The sequential control of these valves was simply achieved bymanipulating the rotating direction and speed of the cartridge. The prototype microfluidic cartridge was 3D printed witha FDM printer and experimentally tested. Anthracene, which was one of the Polycyclic Aromatic Hydrocarbons (PAHs),was used as the target analyte and tested. C18 was used as the stationary sorbent and fluorescence method was adoptedto detect anthracene. The concentrations of the anthracene testing samples were 1μg/L (1ppb), 5μg/L, and 10μg/L(anthracene/water), respectively. The volumes of testing sample and eluent (nonane) were 10mL and 1mL respectively.Experiments showed that 7 times of enrichment of the original samples were achieved. This centrifugal SPE systemcould be potentially used as a portable on-situ pollutant enrichment and detection device.