Continuous infrared laser irradiation decreased membrane capacitance of neuron cell

摘要

Infrared laser had been proved their effectiveness on injury repairing, synchronize embryos and neural regulation etc.Pulsed laser with wavelengths > ～1.5μm was used in controlling nerves in vitro, such as stimulation or block actionpotentials. Theoretical analysis demonstrated that whether enhancement or inhibition could be ascribed to the thermaleffect induced by the pulsed laser. A membrane capacitance mediation mechanism had been proved to act as achromophore in the mediation this thermal effect with the cell function. Here, we explored a continuous infrared laserirradiation situation, which was a case in clinic. A continuous near infrared laser with a wavelength centered at 980nmwas employed to irradiate a neuron cell in vitro. And a patch clamp technique was employed to record a whole cellcurrent. The whole cell current was formed by a compensated capacitance current, a rapid voltage-dependent inwardsodium current, and a slowly voltage-dependent outward-rectifier potassium current. After a 36s irradiation, thepotassium current was changed not that much, but the sodium current was decreased in its peak current. We analyzed thecompensated capacitance current and found that the infrared irradiation decreased the membrane capacitance also.Analysis demonstrated that this can also be related to temperature raising gradient. Our results support that a nearinfrared continuous laser change neurons activities by reducing its membrane capacitance.