Two-photon laser scanning microscopy integrated with light stimulus　system

摘要

We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In thissystem, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diodeserves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. Thecontrol signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulatingthe intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to thesample in real time.