Through Cuticle Neural Imaging of Drosophila Melanogaster with Multiphoton Microscopes

摘要

Multiphoton neural imaging of Drosophila melanogaster required the removal of dorsal head capsule cuticle, prohibiting longitudinal imaging studies (> 5hrs). Here we demonstrate two-and three-photon imaging on cuticle-intact Drosophila brains with dorsal air-sacs removed.