Rapid vertical tissue imaging with clinical multiphoton tomography

摘要

Multiphoton imaging commonly relies on laser-scanning setups which quickly image horizontal sections (x y images) by pixelwise scanning a sample region with focused laser pulses. Different horizontal planes are imaged by adjusting the distance relative between focusing optics and sample. However, in many cases actually a visualization of vertical sections is desired that then can only be obtained indirectly from time-consuming acquisition and processing of complete volume scans. We present a modified multiphoton tomograph for clinical in vivo and ex vivo tissue imaging with direct and fast x-z-imaging capability and exemplify different applications spanning from visualizing anatomic structures to substance penetration studies. The fast x-z imaging is realized by synchronizing the scanning-mirror movement with the tuning of the relative distance between sample and focusing optics