COMPUTATIONAL FLUID DYNAMICS (CFD) MODELLING AND EXPERIMENTAL CONFIRMATION OF HOLLOW FIBER TANGENTIAL FLOW FILTRATION (HFTFF) AND ALTERNATING TANGENTIAL FLOW FILTRATION (ATF) IN A PERFUSION BIOREACTOR

摘要

Hollow fiber tangential flow filtration (HFTFF) and Alternating tangential flow filtration (HFATF) are technologies of choice in continuous (perfusion) bioreactor operations. A major drawback of these technologies is membrane fouling and associated reduction in membrane permeability. Membrane fouling leads to a gradual decline in trans-membrane flux and the sieving of the protein product. Additionally, experimental data suggests that under otherwise similar conditions protein sieving may be different in TFF vs. ATF, indicating that flow behavior patterns in the two technologies may be different.