Sampling was conducted at multiple locations, office, hallway, class rooms and outdoors to determine the impact of distance and human presence on the microbiome of the indoor air The outdoor signature. Bacillus sp. on the microbiome indoors decreased as the distance increased inwards. The concentration of microbiome was affected by the presence/absence of occupants. Human activities caused the increase of bacterial levels. However, bacterial diversity was not significantly influenced by the occupancy because human activities were not diverse. Higher levels of Micrococcus sp., which is related to human skin, were measured in two classrooms when they were occupied. Staphylococcus sp. was also detected. Particle size distribution showed dominance of smaller sizes < 5 urn for both occupied and unoccupied scenarios but increased when the classrooms were occupied.
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