首页> 外文会议>ASME international mechanical engineering congress and exposition >OSTEO-INDUCTION OF HUMAN ADIPOSE DERIVED STEM CELLS CULTURED ON POLY (L-LACTIC ACID) SCAFFOLDS PREPARED BY THERMALLY INDUCED PHASE SEPARATION METHOD
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OSTEO-INDUCTION OF HUMAN ADIPOSE DERIVED STEM CELLS CULTURED ON POLY (L-LACTIC ACID) SCAFFOLDS PREPARED BY THERMALLY INDUCED PHASE SEPARATION METHOD

机译:热诱导相分离法在聚(L-乳酸)支架上培养人脂肪干细胞的成骨诱导

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Bio-degradable Poly (l-lactic acid) (PLLA) scaffolds synthesized using thermally induced phase separation (TIPS) method was used to load cryo-preserved human adipose derived stem cells (hASCs). To make the scaffolds, PLLA-Dioxane solutions were formed by dissolving PLLA in 1,4-Dioxane with three different compositions (wt/vol). These PLLA-Dioxane solutions, frozen in three different cooling rates were lyophilized at 0.037bar and -70°C for 48hrs resulting in porous PLLA scaffolds. Based on the porosity, pore size and compressive strength, a suitable scaffold was chosen to investigate its bio-compatibility and osteo-inductive potential.
机译:使用热诱导相分离(TIPS)方法合成的生物可降解聚(l-乳酸)(PLLA)支架用于加载冷冻保存的人脂肪来源的干细胞(hASC)。为了制备支架,通过将PLLA溶解在具有三种不同组成(wt / vol)的1,4-二恶烷中来形成PLLA-二恶烷溶液。将以三种不同冷却速率冷冻的这些PLLA-二恶烷溶液在0.037bar和-70°C下冻干48小时,从而得到多孔PLLA支架。根据孔隙率,孔径和抗压强度,选择合适的支架来研究其生物相容性和骨诱导潜力。

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