DEVELOPMENT OF NOVEL 3D SCAFFOLDS WITH EMBEDDED CORE-SHELL NANOPARTICLES FOR NERVE REGENERATION

摘要

Neural tissue engineering has emerged as a promising alternative to address various nerve injuries. Particularly, advancement in both 3D biomimetic scaffold fabrication strategies and nanotechnology has inspired this field into a new era. In this study, we fabricated a novel 3D biomimetic scaffold, which has tunable porous structure and embedded core-shell nanoparticles with neurogenic factor delivery system, using stereolithography (SL) based 3D printing and core-shell electrospraying techniques. Our results indicated that scaffolds with higher porosity significantly improve PC-12 neural cell adhesion compared to ones with smaller porosity. Furthermore, scaffolds embedded bovine serum albumin (BSA) containing nanoparticles showed an enhancement in cell proliferation relative to bared control scaffolds. In addition, confocal microscopy images illustrated that the scaffold with nerve growth factor (NGF) nanoparticles increased the length of neuritis and directed neurite extension of PC-12 cells along the fiber. The results in this study demonstrate the potential of this 3D scaffold in improving neural cell function and nerve growth.