The numerical simulation and goniometric measurements of cells light scattering based on Mie Theory

摘要

Optical diagnostic technique, due to its rapid and non-invasive for the diagnosis diseases at the cellular level, can be performed in vivo and allow for real-time diagnosis. While light scattering method is capable of characterizing the structural properties of tissue at the cellular and subcellular scale. In this paper, the spherical models of cells light scattering were established based on Mie, and the distribution curves of scattering intensity in the range of 0～180 degrees were got to explore change rule of cells light scattering information at the molecular level. Also, a platform for experiments used to measure the light scattering information of cells was built to get the change rule of cells light scattering information in wide angular range. And the particle size distribution (PSD) of cells was got by the inversion algorithm. A comparative analysis between numerical simulation and goniometric measurements revealed that the forward-scattering and side-scattering were influenced by the particle size of cells and relative index of refraction between cells and surrounding media. It could also be concluded that it was necessary to get and analyze the light scattering information of larger scattering angle range, which may be related to the intracellular organelles and nucleus.