Hydrophobin-glucose interaction monitored by porous silicon optical multi-layers hybrid interfaces for sugar-proteins interaction monitoring

摘要

Amphiphilic proteins, which self-assemble at solid-liquid interface in nanometricbiolayer, such as hydrophobins, can be used as multifunctional film to passivate porous silicon dioxide, and also sense glucose. Several porous silicon dioxide optical transducers (rugate filter, Thue-Morse sequence, andmicrocavity) have been protein-modified and tested in monitoring hydrophobins-glucose binding. A simple, easy-to-integrate technique, such as water contact angle, is able to reveal sugar presence at 1.2 mg/mL, whereas spectroscopic reflectometry fails. Fluorescence measurements confirm protein layer-glucose interaction.