Effect of Intermittent Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

摘要

Fluid shear stress (FSS) is known to regulate the proliferation and differentiation of mesenchymal stem cells (MSCs) various signaling pathways; therefore it is widely used in the research of tissue engineering. Our previous study has shown that Continuous FSS treatment at 10 dyne/cm2 for 24 h could promote cardiomyogenic differentiation of rBMSCs. The aim of this study is to investigate the effects of intermittent FSS on cardiomyogenic differentiation of rat BMSCs (rBMSCs) in vitro. rBMSCs were isolated from marrow of rat femur and tibia using density gradient centrifugation combined with adhesion method. A parallel-plate flow chamber was used to shear cultured rBMSCs. The results demonstrated that intermittent FSS treatment alone induced cardiomyogenic differentiation of rBMSCs, as confirmed by the expression of cardi-omyocyte-related markers at both mRNA and protein levels. Furthermore, there were no significant differences in the expression of cardiomyocyte-related markers between cells sheared intermittently versus continuously. These results suggest that FSS is an important factor affecting cardiomyogenic differentiation of rBMSCs. Fluid flow may be beneficial component of a bioreactorstrategy to form more orientated differentiated stem cells suitable for regenerative medicine.