In situ visualization of collagen fiber produced by cultured osteoblasts using sensitive second-harmonic-generation microscopy equipped with a 10-fs mode-locked Ti:Sapphire laser

摘要

In this paper, we constructed sensitive second-harmonic-generation (SHG) microscopy equipped with a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in situ.