Cloning and homologous analysis of a partial lipase gene in Staphylococcus caprae TCCC 11546: Partial lipase gene sequencing of S. caprae

摘要

Lipase activity is popular in staphylococci. We described here that a 781-bp consensus lipase gene sequence of Staphylococcus caprae TCCC 11546, which encoded a deduced polypeptide of 260 amino acid residues, was cloned using the degenerate primers. The amino acid sequence alignment and the phylogenetic tree revealed that this 260-amino acid partial lipase of S. caprae was highly homologous from 55.0% to 98.8% among other 11 conserved lipase stretches from other 11 Staphylococcus species. Moreover, two amino acid residues, Ser39 and Asp230, were identified in the putative lipase catalytic triad, the consensus ‘P-loop’ motif (-[AG]-x4-G-K-[ST]-) was also found in this partial lipase stretch.