Application of Phosphorescence Quenching Technique to In Vivo Microvascular Oxygen Measurements

摘要

To clarify the oxygen transport across the microvessel and oxygen distribution in the skeletal muscle,we have newly designed an intravital laser microscopy with oxygen-dependent quenching of phosphorescence technique.This microscope was applied to measure the intra-and peri-vascular partial oxygen pressure (pO2) in rat cremaster muscle.A significant drop in pO2 along the arterioles and a large pO2gradient at the blood/tissue interface of arterioles were found.These findings suggest that oxygen is suppliedfrom arterioles to surrounding tissues and arteriolar wall O2 consumption would be high.