Molecular characterization of spittlebug Mahanarva spectabilis ( Distant,1909:Hemiptera: Cercopidae) populations occurring in 'Legal Amazon' in Brazil

摘要

Recent reports have indicated the occurrence of the spittlebug M ahanarv a in pastures of Brachiaria briz antha cv.′Marandú′ in Cerrados and transition areas, located in the.Legal Amazon" in Brazil.Paula‐Moraes et al.(2006) identified this spittlebug species as M ahanarv a spectabilis Distant ( 1909 ).Since then studies related to the aspects of the M ahanarv a bioecology, economic impact, its geographic distribution, as well as information on the genetic inter and intra‐specific variability have been done.Considering this, the correct identification of the nymphs and adults of the species occurring in different areas has extreme importance.Molecular markers have been used as a tool to support taxonomy studies and to access the genetic diversity of populations of insects and plants.So, the objective of this study was to validate a DNA exctration protocol of M.spectabilis stored in different conditions.It also had the objective to analyze the genetic similarity between recollected nymphs and adults of M.spectabilis in different sites.