Research on the sterilization method for endophytic Fungi of tall fescue seeds

摘要

Seed explant is usually used in tissue culture of tall fescue.However, the fungal endophyte Neoty phodium coenophialum that symbioses with tall fescue may cause high contamination rates in the course of callus culture ( Hinton & Bacon, 1985;Wang & Ge,2005).So,an effective sterilization method of seeds is necessary for establishment of regeneration systems of tall fescue using tissue culture.