Fluorescence lifetime correlation spectroscopy

摘要

Fluorescence lifetime correlation spectroscopy (FLCS) is a recently developed method which combines the conventional fluorescence correlation spectroscopy (FCS) and time correlated single photon counting (TCSPC). It enables to perform a signal separation of the species which possess different lifetime. Particular diffusion components of a mixture of more fluorescent species can be thus separated. Moreover, the detector afterpulsing can be suppressed by FLCS.