Evaluation of stabilizing effect for several monoclonalantibody immobilized quartz crystal microbalance by stabilizer reagents

摘要

We tested five stabilizers for remaining immunologic activity of anti-dinitrophenol (DNP), anti-C-reactive protein (CRP), and anti-2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) antibody immobilized QCM under several storage conditions. Investigated nine stabilizers were as following; 0.25% BlockAce/sup /spl reg// and StabilGuard/sup /spl reg// as commercial available reagents, 0.2% glycerin and 0.2% bovine serum albumin (BSA) as conventional stabilizers, 0.2% MPC copolymer stabilizer as developed reagent, and PBS solution as blank reagent. According to the experimental results, we found MPC copolymer (NOF Corp., Japan) coated QCM showed highly immunologic activity through specific antigens and their antibodies after the heat acceleration test and long-term storage.