Immune-microassay with optical proteinchip for protein detection

摘要

An optical proteinchip upon antigen-antibody affinity and the chip analysis system have been used in immune analysis. The chip is processed with chip design, surface patterning, surface modification, ligand immobilization to form a microarray with many immune probes-one kind of antigen (or antibody) occupying one unit in the microarray and many such units in matrix. In microreactor of bio-molecule interaction, when ligand met its receptor, they would bind into bio-specific complex, which resulted in the thickness of the probe surface layer (surface concentration of antigen-antibody) increasing. The variation of protein pattern on the chip surface corresponding to the ligand immobilization and protein interactions was screened with optical imaging ellipsometry. A nonlabeling microassay for protein detection was realized with antigen-antibody affinity and nondisturbance optical sampling, which was available for the direct test of crude samples, such as serum, etc. Some application results in immune analysis were presented also.