STUDY ON THE THREE-PHASE EXTRACTION OF PENICILLIN G WITH A SINGLE-STEP METHOD

摘要

A novel three-phase extraction with a single-step method is developed for the extraction of penicillin G from the whole fermentation broth and filtered supernatant. The three phases are composed of organic solvent and two aqueous phases, which were traditionally composed of polymer and corresponding polymer or salts. The organic solvent must have low solubility compared to the polymers. In the extraction process, the extraction and purification was finished with a single step in the three phases. The color grade, water content and contamination index of butyl acetate in the first circle are used for estimating the extraction ratio and purification degree. For average results for the filtrate of fermentation broth, the color grade is 2, the water content is less than 1.03 and the contamination index is less than 0.3. For whole-broth processing, the color grade is 5, the water content is less than 1.2 and the contamination index is less than 0.28. The extraction processes, such as flocculation, demulsion, decoloration and freezing dehydration, are integrated into the three-phase extraction. The novel three-phase extraction can be recognized as an advanced method for complex systems, such as the purification of bioproducts from fermentation broths.