Liposome-mediated dna vaccination: the effect of vesicle composition

摘要

Studies from this laboratory (1,2) have shown that plasmid DNA can be quantitatively entrapped into liposomes using the dehydration-rehydration procedure (DRV liposomes) (3). Such DNA-containing DRV have been found to effectively protect DNA from deoxyribonuclease degradation (1,2) and to be more efficient in raising an immune response to the protein encoded by the DNA than either naked DNA or DNA complexed with preformed DRV (4). Here we report the effect of liposomal lipid composition on the efficiency of liposomal DNA vaccination and present data on the characterisation of the most efficient composition.