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Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification

机译:单分子鉴定中的时间相关单光子计数和荧光相关光谱的比较

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Currently, two methods of detection and identification of single molecules are widely used: fluorescence correlation spectroscopy (FCS) and time-correlated single photon counting (TCSPC). We present a thorough theoretical analysis of the error rates for identifying single molecules according to their diffusion coefficients (using FCS), and to their fluorescence lifetimes (using TCSPC). In most cases, the error rate using TCSPC is much lower. TCSPC is thus proven to be more versatile for analyzing single molecule events. The study is significant for a broad range of ultrasensitive fluorescence detection applications.
机译:目前,两种检测和鉴定方法是广泛使用的:荧光相关光谱(FCS)和时间相关的单光子计数(TCSPC)。 我们对根据其扩散系数(使用FCS)和它们的荧光寿命(使用TCSPC)来呈现识别单分子的误差率的彻底理论分析。 在大多数情况下,使用TCSPC的错误率要低得多。 因此,TCSPC被证明是更通用的用于分析单个分子事件。 该研究对于广泛的超敏感荧光检测应用具有重要意义。

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