A modified fast laser-Doppler flowmetry to measure the pulsatile microcirculation in rats

摘要

A fast laser-Doppler flowmetry (LDF) is modified from a commercial one to measure the pulsatile microcirculation in rats. The time constant (TC) of the integrator in the analogue circuit of the LDF was modified; the frequency response of the modification was simulated by PSPICE to estimate the high 3 dB frequency. The coefficients of variance of 32 2-second ensembles of flux in the calibration standard measured by the commercial LDF and the modified one were less than 2%. The animal tests also clearly demonstrated the pulsatile microcirculation on the renal cortex in the rat.