Detection and analysis of polymerase chain reaction products by mass spectrometry

摘要

Abstract: While molecular weight determination of proteins by matrix- assisted laser desorption/ionization (MALDI) has been quite successful, the measurement of deoxyribonucleic acid polymers in a size range useful for fine-structure genome analysis has proven more difficult, due to decreasing sensitivity and resolution at the higher molecular weights. Tailored cleanup techniques and the use of delayed extraction time-of-flight mass spectrometry are approaches that offer considerable promise for improved MALDI analysis of DNA. !11